Watch the video Adaptive Immunity Part 2 - Cell-Mediated
Cell-mediated immunity is all about T-cells and begins with the activation of the helper T-cell the same way as discussed in the humoral response (which you can review in the video or the section above). To activate a cytotoxic T-cell, the same antigen fragment must also be expressed on a MHC-I receptor (recall that these are on all nucleated body cells). Once this fragment is expressed, a naïve cytotoxic T-cell with a TCR that matches the antigen present on the MHC-I will bind. CD8 expressed by the cytotoxic T-cell acts as a co-receptor and will bind to a portion of the MHC-I as well. The cytotoxic T-cell will then express cytokine receptors. The activated helper T-cell mentioned earlier will bind to receptors expressed on cytotoxic T-cells. This results in activation of the cytotoxic T-cell and causes it to proliferate to form a clone army made up of identical activated cytotoxic T-cells that will all recognize the same antigen. Some of these cells will become activated memory cytotoxic T-cells. Later in time, if the body is exposed to the same antigen, these memory cells can be quickly activated. Activated cytotoxic T-cells will recognize body cells that are infected by viruses and other intracellular pathogens by fragments expressed on their MHC-1 molecule. Cytotoxic T-cells will bind to these infected cells with its TCR. CD8 of the cytotoxic T-cell will bind to the MHC-1 of the infected body cell. After binding, the cytotoxic T-cell releases perforins that will bind to the infected body cell and form pores in its plasma membrane, making it prone to lysis and death. Cytotoxic T-cells also release granzymes that induce apoptosis (programmed cell death) of the infected body cell.
Watch the video Helper T-Cells
There are several kinds of helper T-cells that allow for a more specialized immune response. The differentiation of different types of helper T-cells depends on cytokines released by the APC during the initial activation. If the APC releases IL-12, the helper T-cell will become a T-helper 1 cell or TH1. TH1 specifically releases INF-gamma to macrophages and B-cells. This causes the B-cells to make IgG. TH1 is most effective against intracellular viruses and bacteria as well as autoimmune problems. If the APC releases IL-4, the helper T-cell will become a TH2. TH2 may release IL-4 to B-cells and cause a class switch to IgE. TH2 may also release IL-5 to activate and recruit eosinophils. The final cytokine that TH2 will release is IL-13 to stimulate mucus production in epithelial cells. TH2 is the subtype that is found prominently in individuals suffering from allergies. These allergies are often referred to as atopic if they involve predominantly IgE antibodies. If the APC releases TGF-beta and IL-6, the helper T-cell will become TH17. TH17 releases cytokines to activate monocytes (precursor to macrophages) and neutrophils.
T-regulatory cells (Treg) are also produced by the body. While research is being conducted on them, much is still unknown about these cells. For now, remember that they keep the adaptive immune cells functioning properly as well as preventing them from becoming hyperactive and causing autoimmunity. A Treg cell is made when an APC releases IL-2 and TGF-β.
Helper T-Cells and Treg Cells Image by JS F16
The Four Types of HypersensitivityImage by Becky T F19