CoverModule 1.0. Homeostasis, Membranes, Electrophysiology and ANS1.1. Homeostasis1.1.1. Homeostasis Defined1.1.2. Homeostatic Control Systems1.1.3. Feedback Response Loop1.2. Cell Transport; Water & Solutes1.2.1. Fluid Compartments1.2.2. Osmosis1.2.3. Diffusion of Solutes1.2.4. Active Transport1.2.5. Bulk Transport1.3. Electrophysiology1.3.1. Ions and Cell Membranes1.3.2. Membrane Potentials1.3.3. Graded Potential1.3.4. Action Potentials1.3.5. Refractory Periods1.3.6. Propagation of an Action Potential1.4. The Synapse1.5. The Autonomic Nervous System1.5.1. Organization of the Nervous System1.5.2. Structural Organization of the ANS1.5.3. The SNS and the PNS1.5.4. The Enteric Nervous System1.5.5. Physiology of the ANS1.5.6. Neurotransmitters of the ANS1.5.7. Receptors of the ANS1.5.8. Actions of the Autonomic Nervous System1.5.9. Table of Actions for the SNS and PNS and Some Common DrugsModule 2.0. Skeletal Muscle and Special Senses2.1. Structural Organization of Skeletal Muscle2.2.1. Neuromuscular Junction, Excitation-Contraction Coupling2.2.2. Muscle Contractures and Cramps2.3. Whole Muscle Contraction, Fiber Type, Fatigue and Muscle Pharmacology2.3.1. Motor Units2.3.2. Factors that Influence the Force of Contraction2.3.3. Energy Source for Muscle Contraction2.3.4. Skeletal Muscle Fiber Types2.3.5. Fatigue2.3.6. Muscle Pharmacology2.4. Smooth Muscle2.4.1. Smooth Muscle Contraction2.5. Control of Body Movement2.5.1. Voluntary Control of Muscle2.5.2. Reflexes2.6. Taste and Smell2.6.1. Taste2.6.2. The Sense of Smell2.7. Vision2.7.1. Structure of the Eye2.7.2. Focusing Light on the Retina2.7.3. Converting Light to Action Potentials2.7.4. The Retina2.7.5. Phototransduction2.7.6. Receptive Fields2.8. Hearing and Equilibrium2.8.1. The Nature of Sound2.8.2. The Hearing Apparatus2.8.3. Sound Vibrations to Action Potentials2.8.4. The Sense of Balance and EquilibriumModule 3.0. Cardiovascular System3.1. Structure of the Heart3.1.1. Chambers and Circulation3.2. Cardiac Cell Action Potentials3.2.1. Action Potentials in Cardiac Muscle Cells3.2.2. Action Potentials in Cardiac Autorhythmic cells3.2.3. Cellular Mechanisms of Inotropy and Chronotropy3.3. Electrophysiology of Heart Muscle3.3.1. Heart Conduction System3.3.2. Electrocardiogram (ECG)3.3.3. Abnormal ECG - Current of Injury3.4. The Cardiac Cycle3.4.1. Cardiac cycle3.4.2. Cardiac Measurements and Pressure Volume Loops3.5. Blood vessels and Blood Pressure3.5.1. Arteries and Veins3.5.2. Capillaries3.5.3. Blood Pressure Regulation and Shock3.5.4. Capillary Exchange3.5.5. Myogenic and Paracrine Regulation of Vasoconstriction and Vasodilation3.6. Blood3.6.1. Composition of Blood3.6.2. Hematopoeisis3.6.3. Breaking Down Red Blood Cells3.6.4. HemostasisModule 4.0. Urinary and Respiratory Systems4.1. Function and Structure of the Kidney4.1.1. Urinary System Function4.1.2. Functional Anatomy of the Urinary System4.1.3. The Nephron: Functional Unit of the Kidney4.1.4. The Renal Corpuscle: Bowman's Capsule4.2. Physiology of Urine Production4.2.1. Filtration4.2.2. Renal Clearance4.2.3. Tubular Reabsorption4.2.4. Urine Concentration and Dilution4.2.5. Hormonal Regulation of Urine Production4.3. Acid/Base Balance4.3.1. Buffers4.3.2. Acid/Base Disturbances4.4. The Respiratory System4.4.1. Respiratory System Structure and Function4.4.2. Respiratory Membrane4.4.3. Respiratory pressures and Inspriation/Expiration4.4.4. Alveoli and Surfactant4.4.5. Pneumothorax4.4.6. Pressure-Volume Loops and the Work of Breathing4.5. Gas Exchange and Transport4.5.1. Gas Laws4.5.2. Partial Pressure Gradients in the Lung4.5.3. Alveolar Gas Equation4.5.4. Oxygen and Carbon Dioxide Transport in the Blood4.5.5. Alveolar Ventilation4.5.6. Ventilation/Perfusion Ratio4.6. Chronic Bronchitis and Emphysema4.6.1. Respiratory Control by the Medulla Oblongata4.6.2. Chemicals that Regulate VentilationModule 5.0. Digestive, Endocrine and Reproductive Systems5.1. Functional Anatomy of the Digestive System5.1.1. Layers of the Digestive Tract5.1.2. Enteric Nervous System5.1.3. Organs of the Digestive System5.2. Digestion5.2.1. Carbohydrates5.2.2. Proteins5.2.3. Lipids5.2.4. Lipoproteins5.3. Regulation of Digestive Secretions5.4. Endocrine System5.4.1. Overview of the Endocrine System5.4.2. Hormone Receptors5.4.3. Hormones of the Body5.4.4. Other Hormones: Melatonin and Pheromones5.5. The Hypothalamus and Pituitary Gland5.5.1. Structure and Function of the Hypothalamus and Pituitary Gland5.5.2. The Posterior Pituitary5.5.3. The Anterior Pituitary5.5.4. Growth Hormone5.5.5. Prolactin5.5.6. Thyroid Hormones5.5.7. Adrenal Hormones5.6. Pancreas5.6.1. Insulin and Glucagon5.6.2. Diabetes Mellitus5.7. Reproductive System Anatomy5.7.1. Female Reproductive Anatomy5.7.2. Male Reproductive Anatomy5.7.3. Sexual Development at Puberty5.7.4. Male Reproductive Endocrine Axis5.7.5. Spermatogenesis5.7.6. Female Reproductive System: Oogenesis5.7.7. Ovulation and Fertilization5.7.8. The Ovarian Cycle5.7.9. The Uterine Cycle5.7.10. PregnancyAppendix A. GenderAppendix B. The Placebo EffectB.2.1. The Placebo EffectB.2.2. Examples of the Placebo EffectB.2.3. How do Placebos Work?B.2.4. Are Placebos Ethical?B.2.5. How do we validate actual effectiveness of placebosB.2.6. Tips for evaluating scientific evidenceB.2.7. What about Faith Healings

Neuromuscular Junction, Excitation-Contraction Coupling

An important part of understanding the full story of muscle contraction is understanding how a nerve communicates an electrical signal to a muscle fiber.

Neuromuscular Junction
Image by BYU-I Hannah Crowder S13

You may click the link below to go to a tutorial that will walk you through the image above so that you can orient yourself to all the structures in it.

In order for skeletal muscle fibers to contract, there needs to be an electrical event (an action potential) that is followed by a mechanical event (the contraction of the muscle fiber).

Recall that we have already mentioned the fact that the thick and thin myofilaments slide over each other, like the parts of an extension ladder. The proteins themselves don't shorten. The muscle contraction and shortening occur as the myofilaments grip each other, slide past each other, and shorten the sarcomeres. Thus, this is known as the sliding filament model of muscle contraction. Let's also remember that in order for action potentials to both start and propagate (travel), it is necessary for various ion channels to open and close at just the right time. Some of these ion channels open in response to the binding of a ligand. These types of ion channels are known as ligand-gated ion channels (Nicotinic 1). Other ion channels open in response to a change in voltage (electricity) and are known as voltage-gated ion channels. Now, we'll discuss the sequence of events that occur when an action potential reaches the end of the motor neuron.

  1. An action potential arrives at the axon terminal of a somatic motor neuron. Remember that in skeletal muscle, stimulation by a motor neuron is required for contraction. (This is not necessarily true for smooth and cardiac muscle, but we'll get to that later.) The axon terminal of the motor neuron connects to the muscle fiber via the neuromuscular junction (a synapse).
  2. The arrival of the action potential stimulates voltage-gatedCa2+ channels in the axon's membrane to open, and Ca2+ enters the axon terminal from the extracellular space.
  3. Theaxon terminal contains synaptic vesicles filled with the neurotransmitter acetylcholine (ACh). The increased Ca2+ levels is the signal that stimulates exocytosis (SNARE proteins) of these synaptic vesicles and the release of ACh into the synaptic cleft.
  4. The ACh diffuses across the synaptic cleft, binding to acetylcholine receptors on the ligand-gated ion channels (nicotinic type I) in the sarcolemma of the post synaptic tissue (the muscle fiber). This specialized region of the sarcolemma is known as the motor end plate, and this is the location of the ACh receptors.
  5. ACh binding causesligand-gated Na+/K+ channels to open. These ion channels are permeable to both Na+ and K+. However, more Na+ diffuses into the cell than K+ diffuses out of the cell due to driving forces. The Na+ entering the cell depolarizes the sarcolemma, which then will cause adjacent voltage-gated Na+ channels to open, initiating an action potential that spreads out from the neuromuscular junction. The action potential not only travels across the sarcolemma but also down the T-tubules. Remember, T-tubules are just invaginations (inward protrusions) of the sarcolemma and are filled with extracellular fluid that is high in sodium (Na+) and low in potassium (K+). Also, please notice that the ACh receptors are ligand-gated, but movement of Na+ through them (sphere of influence) causes the closely associated voltage-gated Na+ channels to open, resulting in generation and propagation of an action potential.
  6. While the action potential spreads, let's take a break and describe how the stimulation of the ACh receptors is terminated. Without termination of the signal the signal would continue to induce action potentials. This is done when ACh is cleaved (split) by an enzyme that resides in the cleft called acetylcholinesterase.  This enzyme splits ACh into its two components, acetate (acetyl) and choline, rendering it nonfunctional. The acetate portion of acetylcholine diffuses out of the synaptic cleft. The choline, which is an essential nutrient in the Vitamin B group (B4), is taken up by the axon terminal, where it is recycled to make more acetylcholine. Although our bodies can make choline, we cannot produce enough for our needs and must get it in our diet and recycle what we have.
  7. As the action potential spreads along the sarcolemma and the T-tubules, the resultant change in potential causes other voltage-gated channels in the T-tubule to respond. These channels are called dihydropyridine channels (DHP) or L-type Ca2+ Interestingly, although these channels are Ca2+  channels, they are not used as Ca2+  channels, instead muscles use them simply as voltage sensors. Like all muscles, skeletal muscles depend on intracellular Ca2+  but the source of Ca2+ comes from the sarcoplasmic reticulum not from extracellular sources. The signal to release Ca2+  from intracellular sources comes from the DHP and its mechanically linked interaction with ryanodine receptor channels (RyR), which are calcium channels located in the sarcoplasmic reticulum membrane. These two protein channels span the distance between the T-tubule and the terminal cisternae of the sarcoplasmic reticulum. In response to the change in membrane potential, the DHP channel causes the RyR to open and allows Ca2+ ions to leave the sarcoplasmic reticulum and diffuse into the sarcoplasm. These calcium ions bind to the low affinity binding sites of troponin C, causing it to move the tropomyosin molecules off of the active sites on each G-actin molecule.
  8. Uncovering the active sites allows the myosin heads to bind to the actin binding sites, forming cross bridges. In the resting state, the myosin head is "cocked" which means it is in a high-energy complex. It also has ADP and phosphate (Pi) attached to it. When the myosin head binds to the actin binding site it causes a bond formation which releases energy. Some of this energy is recaptured in the phosphate bond which causes the bond to break. The rest of the energy escapes as heat. Removing the phosphate will cause the myosin hinge region to return (bend) to its resting state. This bending, called the power stroke, forcefully pulls the actin past the myosin. During the power stroke, the ADP is also released from the myosin. Recall that in the arrangement of the thick filaments, half of the myosin molecules are pointing one way, and half are pointing the other. Since the myosin heads on the opposite ends of the thick filaments all pull towards the middle, the overall effect is to cause the sarcomere to shorten. As all of the sarcomeres in the muscle fiber shorten, the entire muscle shortens or contracts.
  9. In order for significant shortening of a skeletal muscle fiber to occur, the myosin heads must detach from the G-actin active sites and then re-attach to a different active site further along the neighboring actin molecule. This is rather similar to the fact that in order to climb a ladder, we must pull ourselves up a rung and then let go and move our hands and feet to higher rungs. In order for this release to occur, each myosin head must bind an ATP molecule. The binding of ATP to the myosin head forms another bond, releasing more energy, but in this case some of the energy is captured in the myosin-actin bond and breaks it, releasing myosin from the actin. The ATPase then hydrolyzes the ATP into ADP and a phosphate group, which causes the head to "re–cock" (the recovery stroke), preparing it for the next power stroke. Hence, binding of ATP allows the head to release, and hydrolysis of ATP re–energizes the head for the next power stroke. During a single muscle cell contraction, each myosin molecule undergoes the entire cross-bridge cycle many times—a process known as cross-bridge cycling. As long as Ca2+ is present and the active sites are exposed, the process will continue.

One other important concept: Using the analogy above, when we climb a ladder, we don't take both hands off of the rungs at the same time. Likewise, when muscles contract, the myosin heads are cycling asynchronously, meaning that they don't all bind actin at the same time, and they don't all release at the same time. At any given time, the 300 or so myosin heads in one thick filament will be at different stages of the cross-bridge cycle.

The movement of myosin heads occurs in two phases:

  1. Thepower stroke occurs when the myosin heads bend and ratchet the actin molecules past the myosin.
  1. Therecovery stroke involves the myosin heads detaching from actin and being cocked back into the high energy position to prepare for the next power stroke.
Crossbridge cycling
Skeletal Muscle Contraction. Downloaded from Wikimedia Commons Dec 2013; Author: OpenStax College; Source: License: Creative Commons Attribution 3.0 Unported license

 (a) Calcium binds to Troponin and active site on actin exposed. (b) Myosin binds to Actin forming cross-bridge. (c) Phosphate released in a power stroke causing the myosin head to pivot and releasing ADP/Phosphate group released. (d) ATP attached to myosin head detaching cross bridge. (e) Myosin head hydrolyzed ATP to ADP and phosphate turning myosin back to ready position

Relaxation can only occur when Ca2+  is removed from the sarcoplasm. Removal of Ca2+  occurs from the actions of SERCA pumps. SERCA stands for "Sarco / Endoplasmic Reticulum Calcium ATPase". These pumps are primary active tranporters that use ATP to pump Ca2+ against a gradients as it moves back into the sarcoplasmic reticulum. They are concentrated at the terminal cisternae. As quickly as calcium escapes through the RYR channel, it is actively put back into the SR. This active pumping is not as quick as diffusion causing the relaxation phase to be longer than the contraction phase. In addition, that activity of the SERCA pumps is the largest consumer of ATP consumption in contracting muscle.

To quickly review, the sliding filament model of muscle contraction explains the fact that when skeletal muscle fibers contract, the individual proteins (actin and myosin) don't shorten. Rather, they slide over each other. ATP is necessary for the detachment of myosin heads from actin. Notice also that when a sarcomere contracts, both the H zone and the light I band shrink in width, while the dark A band doesn't appear to narrow.

Sarcomere Anatomy.  Author:; Site: License: Creative Commons Attribution 4.0 License. Attributions.

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