10 - RNA Modifications
After the RNA molecule is produced by transcription (Part 9), the structure of the RNA is often modified prior to being translated into a protein product. These modifications to the RNA molecule are called RNA modifications. Most RNA modifications apply only to eukaryotic RNA transcripts.
- Which group of organisms modify their RNA transcripts?
The modifications to eukaryotic RNA transcripts include the following:
- 5’ end capping. The 5’ end capping process involves the attachment of a modified nucleotide called 7-methylguanosine (7-mG) to the 5’ end of pre-mRNA molecules. The added 7-mG is sometimes called the 5’ cap.
- 3’ end polyadenylation. 3’ end polyadenylation involves the addition of a string of adenine (A) nucleotides to the 3’ end of the pre-mRNA molecule. The added sequence of A nucleotides is called the polyA tail.
- RNA splicing. Most eukaryotic genes are split genes, being composed of both intron sequences and exon sequences. For split genes, initial transcription in the nucleus produces a precursor mRNA (pre-mRNA) molecule. This pre-mRNA is then spliced, meaning that introns are removed and discarded (see Figure 10.1). The remaining exon RNA segments are spliced together to produce a mature mRNA molecule that is transported to the cytoplasm of the cell for translation.
- RNA processing. RNA processing involves cutting large precursor RNA transcripts into smaller ones. RNA processing can involve both exonucleases (removing nucleotides from the ends of the RNA transcript) or endonucleases (cleaving the RNA transcript at internal sites). The ribosomal RNA (rRNA) molecules that are essential components within ribosomes (see Part 11) commonly experience RNA processing.
- RNA editing. RNA editing involves changing the nucleotide sequence of a mRNA molecule slightly prior to translation.
- Base modification. Occasionally, nitrogenous bases within the RNA transcript are covalently modified by the addition of chemical groups, such as methyl groups.
- What is the difference between a pre-mRNA and a mature mRNA?
- What is the difference between an intron and an exon?
- What is meant by 5’ end capping?
- What is meant by 3’ end polyadenylation?
5’ End Capping
The 5’ end of the pre-mRNA molecule is modified by the addition of a 7-methylguanosine (7-mG) nucleotide. The process of adding the 7-mG to the pre-mRNA is 5’ end capping. 5’ end capping is the first RNA modification, occurring during transcription. 5’ end capping (see Figure 10.2) involves the following enzymes:
- RNA 5’-triphosphatase. RNA 5'-triphosphatase removes one of the three phosphates from the nucleotide at the 5’ end of the pre-mRNA transcript.
- Guanylyltransferase. Guanylyltransferase cleaves GTP to produce GMP and pyrophosphate (PPi). Guanylyltransferase then attaches the 5’ carbon of the GMP molecule to the 5’ carbon on the nucleotide at the 5’ end of the pre-mRNA transcript. It is important to note that an unusual 5’ to 5’ phosphodiester bond is formed, placing three phosphate groups between these two adjacent nucleotides.
- Methyltransferase. Methyltransferase attaches a methyl group to the added guanine nitrogenous base, producing the 7-mG cap.
The 7-mG cap:
- Serves as a binding site for proteins that transport the mRNA from the nucleus to the cytoplasm of the cell.
- Serves as a recognition site for translation factor proteins that help the ribosome bind to the mRNA. Once the ribosome binds to the mRNA, translation begins (see Part 11).
- Protects the 5’ end of the mRNA transcript from degradation by exonucleases.
- Regulates RNA splicing.
- How does the 7-mG structure contribute to translation?
- Which nucleotide provides the energy for 5’ end capping?
- What is unusual about the covalent bond between 7-mG and the rest of the pre-mRNA molecule?
3’ End Polyadenylation
The 3’ end of the pre-mRNA is modified by the addition of a polyA tail, a string of approximately 250 adenine (A) nucleotides. The process of adding a polyA tail to the mRNA transcript (see Figure 10.3), called 3’ end polyadenylation, involves:
- The detection of two recognition sequences (polyadenylation signal sequences) in the pre-mRNA. Both polyadenylation signal sequences are located near the 3’ end of the pre-mRNA.
- The first polyadenylation signal sequence is 5’-AAUAAA-3’. This polyadenylation signal sequence is recognized by the endonuclease cleavage and polyadenylation specificity factor (CPSF).
- The second polyadenylation signal sequence, enriched in guanine and uracil bases, is called the GU-rich sequence. This GU-rich sequence is the binding site for the cleavage stimulatory factor (CstF) protein.
- When CstF and CPSF bind to their respective polyadenylation signal sequences, CstF activates CPSF.
- The CPSF protein cleaves the pre-mRNA 10-35 nucleotides downstream (farther towards the 3’ end) from the 5’-AAUAAA-3’ sequence. This free 3’ end of the pre-mRNA is then available for the addition of adenine nucleotides.
- Poly(A)-polymerase (PAP) attaches as many as 250 adenine nucleotides to the newly generated 3’ end of the pre-mRNA transcript.
The polyA tail functions as follows:
- The polyA tail protects the 3’ end of the pre-mRNA transcript from exonuclease degradation.
- The polyA tail promotes the transport of the mRNA from the nucleus to the cytoplasm of the cell.
- The polyA tail helps the ribosome bind to the mRNA to initiate translation.
The 3’ end polyadenylation process occurs after 5’ end capping, but prior to RNA splicing. In fact, 3’ end polyadenylation assists in terminating transcription in eukaryotes by the torpedo model (see Part 9).
- How does 3’ end polyadenylation contribute to transcription termination in eukaryotes?
- Describe the functions of CPSF, CstF, and Poly(A)-polymerase during 3' end polyadenylation.
Splicing of Group I and Group II Introns
There are three general mechanisms used by eukaryotes to remove introns from RNA molecules. The group I and group II mechanisms are limited to certain types of eukaryotes or certain organelles in a eukaryotic cell. For example, the group I mechanism removes the introns found in ribosomal RNA (rRNA) molecules in certain protozoa. The group II mechanism removes the introns found in the mRNA and transfer RNA (tRNA) transcripts produced by mitochondrial and chloroplast genes. The spliceosome mechanism is the major mechanism that is used to remove introns from pre-mRNA transcripts in the nucleus of eukaryotic cells.
- Removing group I introns. RNA splicing of group I introns occurs by self-splicing, meaning that the precursor RNA molecule catalyzes the removal of its own intron (see Figure 10.4). These catalytic precursor RNAs molecules are RNA enzymes (ribozymes). The self-splicing of group I introns involves the following mechanism:
- A free guanosine nucleoside (guanine nitrogenous base covalently linked to a ribose sugar) binds to a pocket within the intron. The guanosine nucleoside bound to the intron serves as an enzyme cofactor (i.e., assists the ribozyme in catalysis) for the remaining steps in the reaction.
- A break forms at the junction between the 3' end of the first exon and the 5’ end of the intron. The guanosine nucleoside becomes attached to the 5’ end of the intron.
- The released exon cleaves the junction between the 3’ end of the intron and the 5' end of the second exon.
- A phosphodiester bond is formed that links the first and second exons together, generating a mature RNA molecule. The intron is released and degraded.
- Removing group II introns. RNA splicing of group II introns also occurs by self-splicing, meaning that the precursor RNA is an RNA enzyme that removes its own intron (see Figure 10.5). In other words, pre-mRNAs containing group II introns are also ribozymes. The self-splicing of group II introns involves the following:
- The 2’-OH group of an adenine nucleotide within the intron helps to cleave the junction between the 3’ end of the first exon and the 5’ end of the intron. In this reaction, the adenine nucleotide serves as an enzyme cofactor for the reaction.
- The released exon cleaves the junction between the 3’ end of the intron and the 5' end of the second exon.
- A phosphodiester bond is formed that links the first and second exons, generating a mature RNA transcript. The intron is released and degraded.
- What is a ribozyme?
- Describe the major events that occur in the group I and group II splicing mechanisms.
- What molecules serve as enzyme cofactors in the group I and group II splicing mechanisms?
Removal of Introns by Spliceosomes
Transcription of most structural genes in the nucleus of eukaryotic cells produces pre-mRNA molecules; the removal of the introns within these pre-mRNA molecules involves a large multi-subunit spliceosome complex. The spliceosome binds to recognition sequences within the intron RNA sequence (see Figure 10.6). These intron recognition sequences include:
- The 5’ splice site. The 5’ splice site (also called the donor sequence) is a 5’-GU-3’ at the 5’ end of the intron RNA sequence.
- The branch site. The branch site is an adenine nucleotide (A) near the middle of the intron RNA sequence.
- The 3’ splice site. The 3’ splice site (also called the acceptor sequence) is a 5’-AG-3’ at the 3’ end of the intron RNA sequence.
The spliceosome complex contains multiple subunits; these subunits are called small nuclear ribonucleoproteins or snRNPs (“snurps”). snRNPs are composed of uracil-rich small nuclear RNAs (snRNAs) that act as RNA enzymes (ribozymes) to remove the introns from nuclear pre-mRNA molecules. snRNPs are also composed of proteins that function to stabilize the overall spliceosome complex.
The spliceosome splicing mechanism occurs as follows:
- The U1 snRNP binds to the 5’ splice site within the intron RNA sequence, while the U2 snRNP binds to the branch site adenine within the intron.
- Additional snRNPs called U4, U5, and U6 bind to the intron. These five snRNPs (U1, U2, U4, U5, and U6) form the spliceosome complex.
- The intron loops out bringing the two exon sequences close together.
- The 5’ splice site within the intron is cut by U1, and the 5’ end of the intron is covalently linked to the 2’-OH group of the branch site adenine, forming an RNA loop structure called a lariat.
- The U1 and U4 snRNPs are released.
- The 3’ splice site within the intron is cut by the U5 snRNP.
- A phosphodiester bond is formed that links the two exons together to form the mature mRNA molecule.
- The intron is released along with the U2, U5, and U6 snRNPs.
- Which two splicing mechanisms are found in human cells?
- What are the names of the three sequences found within spliceosome introns?
- Which spliceosome components are ribozymes?
- What are the functions of the U1 and U5 snRNPs?
Identifying Introns Using R Loop Experiments
Introns were initially identified within the β-globin and ovalbumin genes by performing R-loop (hybridization) experiments. These experiments relied on denaturing an isolated DNA molecule that contains a gene, allowing a mRNA molecule to form hydrogen bonds (hybridize) with the template DNA strand, adding the coding strand DNA, which attempts to form hydrogen bonds with the template DNA strand, and finally, examining the resulting nucleic acid structure in an electron microscope. What would such a molecule look like at the end of the R-loop (hybridization) experiment? Below are the results expected from two R-loop experiments, one experiment involving the pre-mRNA (before RNA modifications), the other experiment involving the mature mRNA (after RNA modifications).
- Gene hybridized to the pre-mRNA. The pre-mRNA that has formed hydrogen bonds with the template DNA strand prevents the coding DNA strand from binding. Because the coding DNA strand fails to bind to the template DNA strand, the coding DNA strand loop outs from the RNA-DNA hybrid region. This loop where the coding DNA strand cannot bind to the template DNA strand is called an RNA displacement loop or R loop (see Figure 10.7A).
- Gene hybridized to the mature mRNA. Hybridization between the template DNA strand and the mature mRNA forces the intron DNA sequences from the template DNA strand to loop out, because the mature mRNA lacks intron RNA sequences. Adding the coding DNA strand produces R-loops with an intervening region of double-stranded DNA (i.e., the intron sequences within the template and the coding DNA strands from hydrogen bonds) called an intron loop (see Figure 10.7B).
- Suppose a gene contains four introns and is hybridized with its mature mRNA. How many R loops would be observed in the electron microscope at the end of an R loop experiment? How many intron loops would be observed?
Identifying Introns by Comparing gDNA with cDNA
Introns within genes can also be identified by comparing the length of a genomic DNA (gDNA) version of a gene to the complementary DNA (cDNA) version of the same gene. gDNA is the version of a gene found in the genome; the gDNA version of a gene contains both introns and exons. cDNA is produced in the laboratory by reverse transcription (see Part 8). Reverse transcription converts mature mRNA into a cDNA molecule using the viral enzyme reverse transcriptase. Since the cDNA molecule is produced from the mature mRNA, cDNA molecules contain exons but lack introns. The gDNA version of the gene, which contains introns, will be longer than the cDNA version of the same gene, which lacks introns.
The polymerase chain reaction (PCR) technique (see Part 8) can be used to make billions of copies of the gDNA and the cDNA versions of any gene of interest. The gDNA and cDNA PCR products are then separated by size using agarose gel electrophoresis (see Part 8). The size difference between the gDNA and the cDNA copy of the gene can be easily observed on an agarose gel (see Figure 3.2).
- What is the difference between gDNA and cDNA?
- How can comparing gDNA to cDNA on an agarose gel help you determine that a gene contains introns?
Alternative splicing involves splicing a single type of pre-mRNA molecule in various ways to produce different mature mRNA molecules (see Figure 10.9). Each of these mature mRNAs can then produce slightly different proteins upon translation. These distinct, yet related protein isoforms, all derived from a single gene, can have specialized functions.
Alternative splicing is beneficial in that it allows eukaryotes to carry fewer genes in the genome, permitting a relatively small number of genes the flexibility to encode a vast array of proteins. In humans, it is estimated that 30–60% of the genes in the genome are alternatively spliced. As a result, the human genome, which contains approximately 23,000 structural genes, can produce at least ten times that number of protein products.
One example of alternative splicing involves the splicing of the pre-mRNA molecule for α-tropomyosin, a protein involved in muscle contraction. The α-tropomyosin gene contains 14 exons (13 introns). There are two types of exons within the α-tropomyosin pre-mRNA:
- Constitutive exons. Constitutive exons are exons that are always included in the mature α-tropomyosin mRNAs products of alternative splicing. These exons likely encode amino acid sequences that maintain the general three-dimensional structure of the encoded α-tropomyosin protein.
- Alternative exons. Alternative exons vary between mature α-tropomyosin mRNAs. In one cell type, one combinations of alternative exons are spliced together, in another cell type, a different combination of alternative exons are spliced together. The result is two related proteins that have slightly different functions to meet the unique needs of these two cell types.
- Why is alternative splicing advantageous to eukaryotic cells?
- What are protein isoforms?
- What is the difference between a constitutive exon and an alternative exon?
Patterns of Alternative Splicing
Alternative splicing is regulated by splicing factor proteins. These splicing factor proteins help the spliceosome complex choose which intron splice recognition sites to use during RNA splicing. Different cell types have different splicing factor proteins, allowing different RNA splicing patterns to occur in each cell type. The SR proteins are an example of a group of splicing factor proteins found in animals, including humans.
Here are some common alternative splicing patterns observed in eukaryotic cells:
- Exon Skipping. Some splicing factor proteins act as splice repressors. Splice repressor proteins prevent the spliceosome from recognizing a particular 3' splice site within an intron (see Figure 10.10). When a splice repressor protein blocks a 3’ splice site within an intron, the 3’ splice site in the next intron is chosen for splicing instead, and the intervening exon is removed (exon skipping).
- Alternative 5' and 3' Splice Sites. In addition to the 5’ splice site, the branch site, and the 3’ splice sites discussed earlier, there are other pre-mRNA sequences involved in RNA splicing. These additional sequence elements, often located within a nearby exon, can promote the use of a particular 5’ or 3’ splice site. For example, some potential 5’ or 3’ splice sites in the pre-mRNA are poorly recognized by the spliceosome. In certain cell types, the binding of a splice activator protein to a splice enhancer sequence within a nearby exon promotes the use of these otherwise poorly recognized 5’ or 3’ splice sites (see Figure 10.10). When a splice activator protein binds to a splice enhancer sequence, an exon is included in the mature mRNA (i.e., the exon is not skipped).
- Mutually Exclusive Exons. In some cases, splicing events are coordinated between different cell types to ensure that unique protein isoforms are produced in the two cell types. For example, suppose there are four exons (three introns) in a pre-mRNA molecule. During splicing in one cell type, exon two is consistently retained in the mature mRNA, while exon three is always spliced out. In a different cell type, exon two is always spliced out, while exon three is always retained in the mature mRNA. Exons one and four are found in the mature mRNAs in both cell types and are thus constitutive exons.
Scientists know little about the true complexity of alternative splicing; however, alternative splicing patterns are cell-type and developmental stage specific. Moreover, mutations in human genes often lead to aberrant splicing patterns. This aberrant splicing produces abnormal protein isoforms and ultimately, disease phenotypes.
- What happens when a splice repressor protein binds to the 3’ splice site within an intron?
- What effect would a splice activator protein binding to a splice enhancer sequence have on alternative splicing?
- What is meant by the term mutually exclusive exons?
Fill in the blank:
- __________________ is an endonuclease that releases the pre-mRNA from RNA polymerase II during transcription.
- ____________________ is an enzyme that attaches two nucleotides together via a 5’ to 5’ covalent bond.
- One function of the 7-mG cap is to _______________________________________.
- A __________________________ protein prevents the spliceosome from binding to a 3’ splice site.
- ________________________ is an enzyme that adds adenine nucleotides to the 3' end of a pre-mRNA. These are added in the 5' to 3' direction.
- The Group I intron splicing mechanism uses the nucleoside ______________ as a cofactor during catalysis, while the _______________ intron splicing mechanism uses an adenine nucleotide as a cofactor during catalysis.
- The U2 snRNP binds to the ________________________ site of the pre-mRNA.
- Spliceosome subunits are composed of two components: proteins and _________________.
- ______________________ is a pattern of alternative splicing where one exon is always retained in one cell while that same exon is always skipped in another cell.
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